Review



cav2 2 af488 conjugated  (Bioss)


Bioz Verified Symbol Bioss is a verified supplier
Bioz Manufacturer Symbol Bioss manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Bioss cav2 2 af488 conjugated
    Visualization of the proposed methodology: propagation of action potentials along the axon initiates a cascade of events, culminating in the detection of magnetic fields by fluorescent nanodiamonds (FNDs), particles with magnetic nanosensitive properties. The FNDs selectively bind to <t>Cav2.2</t> calcium channels distributed along the cellular structure. This binding process is facilitated through the functionalization of FNDs with Cav2.2 targeting antibodies, using the strong covalent bond between streptavidin and biotin. This strategic molecular interplay enhances the specificity and efficiency of the approach, which is crucial for sensitivity optimization.
    Cav2 2 Af488 Conjugated, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cav2+2+af488+conjugated/CACNA1B+(N+type)+Polyclonal+Antibody%2C+AbBy+Fluor+488+Conjugated/pmc11551900-149-14-18
    Average 94 stars, based on 2 article reviews
    cav2 2 af488 conjugated - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection"

    Article Title: Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection

    Journal: ACS Applied Materials & Interfaces

    doi: 10.1021/acsami.4c12462

    Visualization of the proposed methodology: propagation of action potentials along the axon initiates a cascade of events, culminating in the detection of magnetic fields by fluorescent nanodiamonds (FNDs), particles with magnetic nanosensitive properties. The FNDs selectively bind to Cav2.2 calcium channels distributed along the cellular structure. This binding process is facilitated through the functionalization of FNDs with Cav2.2 targeting antibodies, using the strong covalent bond between streptavidin and biotin. This strategic molecular interplay enhances the specificity and efficiency of the approach, which is crucial for sensitivity optimization.
    Figure Legend Snippet: Visualization of the proposed methodology: propagation of action potentials along the axon initiates a cascade of events, culminating in the detection of magnetic fields by fluorescent nanodiamonds (FNDs), particles with magnetic nanosensitive properties. The FNDs selectively bind to Cav2.2 calcium channels distributed along the cellular structure. This binding process is facilitated through the functionalization of FNDs with Cav2.2 targeting antibodies, using the strong covalent bond between streptavidin and biotin. This strategic molecular interplay enhances the specificity and efficiency of the approach, which is crucial for sensitivity optimization.

    Techniques Used: Binding Assay

    Validation of the functionalization protocol with a secondary antibody conjugated with Alexa Fluor 405 targeting Cav2.2-functionalized fluorescent nanodiamonds (FNDs). (a) Fluorescence confocal image of green channel displaying secondary antibody AF405 response. (b) Fluorescence confocal image of nanodiamonds exhibiting the NV-fluorescence emission originated inside the particles. (c) Image of the merged channels illustrating the overlap in white color. (d) Co-localization analysis of seven data sets using the Van Steensel’s cross-correlation factor, showing close to perfect co-localization.
    Figure Legend Snippet: Validation of the functionalization protocol with a secondary antibody conjugated with Alexa Fluor 405 targeting Cav2.2-functionalized fluorescent nanodiamonds (FNDs). (a) Fluorescence confocal image of green channel displaying secondary antibody AF405 response. (b) Fluorescence confocal image of nanodiamonds exhibiting the NV-fluorescence emission originated inside the particles. (c) Image of the merged channels illustrating the overlap in white color. (d) Co-localization analysis of seven data sets using the Van Steensel’s cross-correlation factor, showing close to perfect co-localization.

    Techniques Used: Fluorescence

    Interaction of functionalized (f-FNDs) and nonfunctionalized (FNDs) nanodiamonds with SH-SY5Y and dopaminergic neurons. Fluorescence confocal images: (a) SH-SY5Y cells with FNDs. (b) SH-SY5Y cells with f-FNDs. (c) Dopaminergic neurons with FNDs. (d) Dopaminergic neurons with f-FNDs. Channels: blue: DAPI staining (nucleus); green: Cav2.2 AF488 (SH-SY5Y) and anti-TH (dopaminergic); red: FNDs. (e) Absolute number of particles per mm 2 for both cell lines and particles, showcasing higher values with f-FNDs for both cell lines. Scale bar = 10 μm.
    Figure Legend Snippet: Interaction of functionalized (f-FNDs) and nonfunctionalized (FNDs) nanodiamonds with SH-SY5Y and dopaminergic neurons. Fluorescence confocal images: (a) SH-SY5Y cells with FNDs. (b) SH-SY5Y cells with f-FNDs. (c) Dopaminergic neurons with FNDs. (d) Dopaminergic neurons with f-FNDs. Channels: blue: DAPI staining (nucleus); green: Cav2.2 AF488 (SH-SY5Y) and anti-TH (dopaminergic); red: FNDs. (e) Absolute number of particles per mm 2 for both cell lines and particles, showcasing higher values with f-FNDs for both cell lines. Scale bar = 10 μm.

    Techniques Used: Fluorescence, Staining

    Related Articles

    Incubation:

    Article Title: Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection
    Article Snippet: After washing, cells were permeabilized with PBS 0.3% Triton X-100 (Sigma) for 5 min. After washing, a blocking solution composed of PBS 10% FBS was added and left incubating for 1 h at RT. .. Cells were incubated for 1 h at RT with different primary antibodies diluted in blocking buffer:mouse polyclonal β-III tubulin antibody (1:1000; Promega), rabbit polyclonal TH antibody (1:500; Sigma), or 6.66 μg/mL Cav2.2 AF488 conjugated (BS-10490R-BF488, Bioss). .. After the incubation period, on wells where mouse polyclonal β-III tubulin antibody or rabbit polyclonal TH antibody was added, cells were washed 3× with PBS, after which an incubation for 1 h with secondary antibodies, Alexa Fluor 488 goat anti-mouse (1:1000; Invitrogen) or Alexa Fluor 594 goat anti-rabbit (1:1000; Invitrogen), diluted in blocking buffer was performed.

    Blocking Assay:

    Article Title: Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection
    Article Snippet: After washing, cells were permeabilized with PBS 0.3% Triton X-100 (Sigma) for 5 min. After washing, a blocking solution composed of PBS 10% FBS was added and left incubating for 1 h at RT. .. Cells were incubated for 1 h at RT with different primary antibodies diluted in blocking buffer:mouse polyclonal β-III tubulin antibody (1:1000; Promega), rabbit polyclonal TH antibody (1:500; Sigma), or 6.66 μg/mL Cav2.2 AF488 conjugated (BS-10490R-BF488, Bioss). .. After the incubation period, on wells where mouse polyclonal β-III tubulin antibody or rabbit polyclonal TH antibody was added, cells were washed 3× with PBS, after which an incubation for 1 h with secondary antibodies, Alexa Fluor 488 goat anti-mouse (1:1000; Invitrogen) or Alexa Fluor 594 goat anti-rabbit (1:1000; Invitrogen), diluted in blocking buffer was performed.



    Similar Products

    cav2 2 af488 conjugated  (Bioss)
    94
    Bioss cav2 2 af488 conjugated
    Visualization of the proposed methodology: propagation of action potentials along the axon initiates a cascade of events, culminating in the detection of magnetic fields by fluorescent nanodiamonds (FNDs), particles with magnetic nanosensitive properties. The FNDs selectively bind to <t>Cav2.2</t> calcium channels distributed along the cellular structure. This binding process is facilitated through the functionalization of FNDs with Cav2.2 targeting antibodies, using the strong covalent bond between streptavidin and biotin. This strategic molecular interplay enhances the specificity and efficiency of the approach, which is crucial for sensitivity optimization.
    Cav2 2 Af488 Conjugated, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cav2+2+af488+conjugated/CACNA1B+(N+type)+Polyclonal+Antibody%2C+AbBy+Fluor+488+Conjugated/pmc11551900-149-14-18
    Average 94 stars, based on 1 article reviews
    cav2 2 af488 conjugated - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Visualization of the proposed methodology: propagation of action potentials along the axon initiates a cascade of events, culminating in the detection of magnetic fields by fluorescent nanodiamonds (FNDs), particles with magnetic nanosensitive properties. The FNDs selectively bind to Cav2.2 calcium channels distributed along the cellular structure. This binding process is facilitated through the functionalization of FNDs with Cav2.2 targeting antibodies, using the strong covalent bond between streptavidin and biotin. This strategic molecular interplay enhances the specificity and efficiency of the approach, which is crucial for sensitivity optimization.

    Journal: ACS Applied Materials & Interfaces

    Article Title: Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection

    doi: 10.1021/acsami.4c12462

    Figure Lengend Snippet: Visualization of the proposed methodology: propagation of action potentials along the axon initiates a cascade of events, culminating in the detection of magnetic fields by fluorescent nanodiamonds (FNDs), particles with magnetic nanosensitive properties. The FNDs selectively bind to Cav2.2 calcium channels distributed along the cellular structure. This binding process is facilitated through the functionalization of FNDs with Cav2.2 targeting antibodies, using the strong covalent bond between streptavidin and biotin. This strategic molecular interplay enhances the specificity and efficiency of the approach, which is crucial for sensitivity optimization.

    Article Snippet: The sample was washed 3× with PBS, and 40 μL of a 6.66 μg/mL Cav2.2 AF488 conjugated (BS-10490R-BF488, Bioss) solution was added overnight.

    Techniques: Binding Assay

    Validation of the functionalization protocol with a secondary antibody conjugated with Alexa Fluor 405 targeting Cav2.2-functionalized fluorescent nanodiamonds (FNDs). (a) Fluorescence confocal image of green channel displaying secondary antibody AF405 response. (b) Fluorescence confocal image of nanodiamonds exhibiting the NV-fluorescence emission originated inside the particles. (c) Image of the merged channels illustrating the overlap in white color. (d) Co-localization analysis of seven data sets using the Van Steensel’s cross-correlation factor, showing close to perfect co-localization.

    Journal: ACS Applied Materials & Interfaces

    Article Title: Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection

    doi: 10.1021/acsami.4c12462

    Figure Lengend Snippet: Validation of the functionalization protocol with a secondary antibody conjugated with Alexa Fluor 405 targeting Cav2.2-functionalized fluorescent nanodiamonds (FNDs). (a) Fluorescence confocal image of green channel displaying secondary antibody AF405 response. (b) Fluorescence confocal image of nanodiamonds exhibiting the NV-fluorescence emission originated inside the particles. (c) Image of the merged channels illustrating the overlap in white color. (d) Co-localization analysis of seven data sets using the Van Steensel’s cross-correlation factor, showing close to perfect co-localization.

    Article Snippet: The sample was washed 3× with PBS, and 40 μL of a 6.66 μg/mL Cav2.2 AF488 conjugated (BS-10490R-BF488, Bioss) solution was added overnight.

    Techniques: Fluorescence

    Interaction of functionalized (f-FNDs) and nonfunctionalized (FNDs) nanodiamonds with SH-SY5Y and dopaminergic neurons. Fluorescence confocal images: (a) SH-SY5Y cells with FNDs. (b) SH-SY5Y cells with f-FNDs. (c) Dopaminergic neurons with FNDs. (d) Dopaminergic neurons with f-FNDs. Channels: blue: DAPI staining (nucleus); green: Cav2.2 AF488 (SH-SY5Y) and anti-TH (dopaminergic); red: FNDs. (e) Absolute number of particles per mm 2 for both cell lines and particles, showcasing higher values with f-FNDs for both cell lines. Scale bar = 10 μm.

    Journal: ACS Applied Materials & Interfaces

    Article Title: Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection

    doi: 10.1021/acsami.4c12462

    Figure Lengend Snippet: Interaction of functionalized (f-FNDs) and nonfunctionalized (FNDs) nanodiamonds with SH-SY5Y and dopaminergic neurons. Fluorescence confocal images: (a) SH-SY5Y cells with FNDs. (b) SH-SY5Y cells with f-FNDs. (c) Dopaminergic neurons with FNDs. (d) Dopaminergic neurons with f-FNDs. Channels: blue: DAPI staining (nucleus); green: Cav2.2 AF488 (SH-SY5Y) and anti-TH (dopaminergic); red: FNDs. (e) Absolute number of particles per mm 2 for both cell lines and particles, showcasing higher values with f-FNDs for both cell lines. Scale bar = 10 μm.

    Article Snippet: The sample was washed 3× with PBS, and 40 μL of a 6.66 μg/mL Cav2.2 AF488 conjugated (BS-10490R-BF488, Bioss) solution was added overnight.

    Techniques: Fluorescence, Staining